Kruyer, N.S., Sugianto, S., Tickman,
B.I., Alba Burbano, D., Noireaux, Carothers, J.M.,
Peralta-Yahya, P. Membrane augmented cell-free systems: a new
frontier in biotechnology.
ACS Synthetic Biology (2021). paper
Greiss, F., Daube, S.S., Noireaux,
V., Bar-Ziv, R.H. From deterministic to fuzzy decision-making in
artificial cells. Nature
Communications 11(1), 5648 (2020). paper-SI
Yim, S.S., Johns, N.I., Noireaux,
V., Wang, H.H. Protecting linear DNA templates in cell-free
expression systems from diverse bacteria. ACS Synthetic Biology 9,
2851-2855 (2020). paper-SI
Garenne, D., Noireaux, V. Membrane
functions genetically programmed in synthetic cells: a barrier
to conquer. Current Opinion
in Molecular Systems Biology 24, 9-17 (2020). paper-SI
Garenne, D., Noireaux, V. Analysis
of cytoplasmic and membrane molecular crowding in genetically
programmed synthetic cells.
Biomacromolecules 21(7), 2808-2817 (2020). paper-SI
Vonshak, O., Divon, Y., Forste, S.,
Garenne, D., Noireaux, V., Lipowski, R., Rudorf, S., Daube,
S.S., Bar-Ziv, R.H. Programming multi-protein assembly by gene
brush patterns and two-dimensional compartment geometry. Nature Nanotechnology
15(9), 783-791 (2020). paper-SI
Noireaux, V., Liu, A.P. The new age
of cell-free biology. Annual
Review of Biomedical Engineering 22, 51-77 (2020). paper-SI
Garenne, D., Libchaber, A., Noireaux,
V. Membrane molecular crowding enhances MreB polymerization to
shape synthetic cells from spheres to rods. Proc. Nat. Acad. Sci. USA
117, 1902-1909 (2020). paper-SI
Wandera, K.G., Collins, S.P., Wimmer,
F., Marshall, R., Noireaux, V., Beisel, C.L. An enhanced assay
to characterize anti-CRISPR proteins using a cell-free
transcription-translation system. Methods 172, 42-59 (2020). paper-SI
Marshall, R., Beisel, C.L., Noireaux,
V. Rapid Testing of CRISPR Nucleases and Guide RNAs in an E.
coli Cell-Free Transcription-Translation System. STAR Protocols 1 (2019). paper-SI
Agrawal, D.K., Marshall, R.,
Noireaux, V., Sontag, E.D. In vitro implementation of robust
gene regulation in a synthetic biomolecular integral controller.
Nature Communications
10, 5760 (2019). paper-SI
Yim, S.S., Johns, N.I., Park, J.,
Gomes, A.L., McBee, R.M., Richardson, M., Ronda, C., Chen, S.P.,
Garenne, D., Noireaux, V., Wang, H.H. Multiplex transcriptional
characterizations across diverse bacterial species using
cell-free systems. Molecular
Systems Biology 15, e8875 (2019). paper-SI
Marshall, R., Noireaux, V.
Quantitative modeling of transcription and translation of an
all-E. coli cell-free system. Scientific
Reports 9, 11980 (2019). paper-SI
Garamella, J., Majumder, S., Liu,
A.P, Noireaux, V. An Adaptive Synthetic Cell Based on
Mechanosensing, Biosensing, and Inducible Gene Circuits. ACS Synthetic Biology 8,
1913-1920 (2019). paper-SI
Izri, Z., Garenne, D., Noireaux, V.,
Maeda, Y.T. Gene Expression in on-Chip Membrane-Bound Artificial
Cells. ACS Synthetic Biology
8, 1705-1712 (2019). paper-SI
Garenne, D., Beisel, C.L., Noireaux,
V. Characterization of the all-E. coli transcription-translation
system myTXTL by mass spectrometry. Rapid Communication in Mass Spectrometry 33,
1036-1048 (2019). paper-SI
Garamella, J., Garenne, D., Noireaux,
V. TXTL-based approach to synthetic cells. Methods in Enzymology 617,
217-239 (2019). paper-SI
Westbrook, A., Tang, X., Marshall,
R., Maxwell, C.S., Chappell, J., Agrawal, D.K., Dunlop, M.J.,
Noireaux, V., Beisel, C.L., Lucks, J., Franco, E. Distinct
timescales of RNA regulators enable the construction of a
genetic pulse generator. Biotechnology
and Bioengineering 116, 1139-1151 (2019). paper-SI
Garenne, D., Noireaux, V. Cell-free
transcription-translation: engineering biology from the
nanometer to the millimeter scale. Current Opinion in Biotechnology 58, 19-27
(2019). paper-SI
Condon, A., Kirchner, H., Lariviere,
D., Marshall, W., Noireaux, V., Tlusty, T., Fourmentin, E. Will
biologists become computer scientists? EMBO Reports 19, e46628
(2018). paper-SI
Kechkeche, D., Cao, E., Grazon, C.,
Caschera, F., Noireaux, V., Baron Niel, M.L., Dubertret, B.
Semiconductor Nanoplatelets: A New Class of Ultrabright
Fluorescent Probes for Cytometric and Imaging Applications. ACS Applied Materials and
Interfaces 10, 24739-24749 (2018). paper-SI
Marshall, R., Noireaux, V. Synthetic
Biology with an All E. coli TXTL System: Quantitative
Characterization of Regulatory Elements and Gene Circuits. Methods in Molecular Biology
1772, 61-93 (2018). paper-SI
Sakamoto, R., Noireaux, V., Maeda,
Y.T. Anomalous Scaling of Gene Expression in Confined Cell-Free
Reactions. Scientific Reports
8, 7364 (2018). paper-SI
Agrawal, D.K., Tang, X., Westbrook,
A., Marshall, R., Maxwell, C.S., Lucks, J., Noireaux, V.,
Beisel, C.L., Dunlop, M.J, Franco, E. Mathematical Modeling of
RNA-Based Architectures for Closed Loop Control of Gene
Expression. ACS Synthetic
Biology 18, 1219-1228 (2018). paper-SI
Marshall, R., Garamella, R.,
Noireaux, V., Pierson, A. High-Throughput microliter-sized
cell-free transcription-translation reactions for synthetic
biology applications using the Echo 550 liquid handler. Labcyte Application Note
APP-G124 (2018). paper-SI
Maxwell, C.S., Jacobsen, Marshall,
R., Noireaux, V., Beisel, B.L. A detailed cell-free
transcription-translation-based assay to decipher CRISPR
protospacer-adjacent motifs. Methods
(2018). paper-SI
Marshall, R., Maxwell, C.S., Collins,
S.P., Jacobsen, T., Luo, M.L., Begemann, M.B., Gray, B.N.,
January, E., Singer, A., He, Y., Beisel, B.L., Noireaux, V.
Rapid and scalable characterization of CRISPR technologies using
an E. coli cell-free transcription-translation system. Mol. Cell 69, 146-157
(2018). paper-SI
Klocke, M., Garamella, J., Noireaux,
V., Franco, E. Engineering DNA nanotubes for resilience in an E.
coli TXTL system. Synthetic
Biology 3(1) ysy001 (2018). paper-SI
Rustad, M., Eastlund, A., Jardine,
P., Noireaux, V. Cell-free TXTL synthesis of infectious
bacteriophage T4 in a single test tube reaction. Synthetic Biology 3(1)
ysy002 (2018). paper-SI
Poolman, B., Noireaux, V. Synthetic
Biology: from engineering living systems to the bottom-up
construction of synthetic Cells. Mighty Microbes, the amazing world of microorganisms
ISBN 978-90-816644-4-8 (2017). paper
Liu, A.P., Noireaux, V. Synthetic
cells have senses too. Research
Features Issue 120, ISSN 2399-1534 (2017). paper
Tayar, A., Karzbrun, E., Noireaux,
V., Bar-Ziv, R. Synchrony and pattern formation of coupled
genetic oscillators on a chip of artificial cells. Proc. Nat. Acad. Sci. USA
114(44) 11609-11614 (2017). paper-SI
Rustad, M., Eastlund, A., Jardine,
P., Noireaux, V. Synthesis of Infectious Bacteriophages in an E.
coli-based Cell-free Expression System. J Vis Exp (126) (2017). paper-SI
Dervaux, J., Noireaux, V., Libchaber,
A.J. Growth and Instability of a Phospholipid Vesicule in a Bath
of Fatty Acids. European
Physical Journal Plus 132: 284 (2017). paper-SI
Majumder, S., Garamella, J.,
Noireaux, V., Liu, A.P. Cell-sized Mechanosensitive and
Biosensing Compartment Programmed with DNA. ChemComm 53, 7349-7352
(2017). paper-SI
Marshall, R., Maxwell, C.S., Collins,
S.P., Beisel, C.L., Noireaux, V. Short DNA Containing Chi Sites
Enhances DNA Stability and Gene Expression in E. coli Cell-free
Transcription-Translation Systems. Biotechnology and Bioengineering 114(9),
2137-2141 (2017). paper-SI
Noireaux and others. Cell-free
Molecular Dissection. Cell
Systems (2017). voices
Schlesinger, O., Chemla, Y.,
Heltberg, M., Ozer, E., Marshall, R., Noireaux, V., Jensen,
M.H., Alfonta, L. Tuning of Recombinant Protein Expression in
Escherichia coli by Manipulating Transcription, Translation
Initiation Rates, and Incorporation of Noncanonical Amino Acids.
ACS Synthetic Biology 6,
1076-1085 (2017). paper-SI
Schenkelberger, M, Shanak, S.,
Finkler, M., Worst, E.G., Noireaux, V., Helms, V., Ott, A.
Expression regulation by a methyl-CpG binding domain in an E.
coli based, cell-free TX-TL system. Phys. Biol. 14, 026002 (2017). paper-SI
Jewett, M.C., Noireaux, V. Synthetic
Biology: Tailor-Made Genetic Code. Nature Chemistry 8(40), 291-292 (2016). paper
Garamella, J., Marshall, R., Rustad,
M., Noireaux, V. The all E. coli cell-free TX-TL toolbox 2.0: a
platform for cell-free synthetic biology. ACS Synthetic Biology doi:
10.1021/acssynbio.5b00296 (2016). paper-SI
Caschera, F., Noireaux, V.
Compartmentalization of an all E. coli cell-free expression
system for the construction of a minimal cell. Artificial Life 22, 1-11
(2016). paper
Worst, E.G., Exner, M.P., De Simone,
A., Schenkelberger, M., Noireaux, V., Budisa, N., Ott, A.
Residue-specific incorporation of noncanonical amino acids into
model proteins using an Escherichia coli cell-free
transcription-translation system. J Vis Exp (114), e54273, doi:10.3791/54273
(2016). paper
Noireaux, V. Construction de cellules synthetiques: du
fondamental au pratique. Medecine/Sciences
31(12), 1126-1132 (2015). paper
Karzbrun, E, Tayar, A., Noireaux, V., Bar-Ziv, B.H.
Propagating gene expression fronts in a 1D coupled system of
artificial cells. Nature
Physics doi: 10.1038/NPHYS3469 (2015). paper-SI
Worst, E.G., Exner, M.P., De Simone, A.,
Schenkelberger, M., Noireaux, V., Budisa, N., Ott, A.
Cell-free expression with the toxic amino acid canavanine. Bioorganic & Medical
Chemistry Letters 1, 25(17), 3658-60 (2015). paper
supp
Takahashi, M., Hayes, C.A., Chappell, J., Sun, Z.Z.,
Murray, M.M., Noireaux, V., Lucks, J. Characterizing and
prototyping genetic networks with cell-free
transcription–translation reactions. Methods doi:
10.1016/j.ymeth.2015.05.020 (2015). paper
supp
Chemla, Y,, Ozer, E., Schlesinger, O., Noireaux, V.,
Alfonta, L. Genetically expanded cell-free protein synthesis
using endogenous pyrrolysyl orthogonal translation system. Biotechnology and Bioengineering
doi: 10.1002/bit.25587 (2015). paper
supp
Caschera, F., Noireaux, V. Preparation of amino acid
mixtures for cell-free expression systems. Biotechniques 58, 40-43
(2015). paper
supp
Caschera, F., Noireaux, V. A cost-effective
polyphosphate-based metabolism fuels an all E. coli cell-free
expression system. Metabolic
Engineering 27, 29-37 (2015). paper
supp
Takahashi, M., Chappell, J., Hayes, C.A., Sun, Z.Z.,
Singhal, V., Spring, K., Al-Khabouri, S., Fall, C., Noireaux,
V., Murray, M.M., Lucks, J. Rapidly characterizing the fast
dynamics of RNA genetic circuitry with cell-free
transcription-translation (TX-TL) systems. ACS Synthetic Biology
4(5), 503-515 (2015). paper
supp
Caschera, F., Noireaux, V. Integration of biological
parts towards the synthesis of a minimal cell. Current Opinion in Chemical
biology 22, 85-91 (2014). paper
Karzbrun, E, Tayar, A., Noireaux, V., Bar-Ziv, B.H.
Programmable on-chip DNA compartments as artificial cells. Science 345(6198),
829-832 (2014). paper
supp
Siegal-Gaskins, D., Tuza, Z., Kim, J., Noireaux, V.,
Murray, M.M. Resource usage and gene circuit performance
characterization in a cell-free ‘breadboard‘. ACS Synthetic Biology
3(6), 416-425 (2014). paper
supp
Coutable, A., Thibault, C., Chalmeau, J., Francois,
J.M., Vieu, C., Noireaux, V., Trevisiol, E. Preparation of
tethered-lipid bilayers on gold surfaces for the incorporation
of integral membrane proteins synthesized by cell-free
expression. Langmuir
30(11), 3132-3141 (2014). paper
supp
Coutable, A., Randrianjatovo, I., Noireaux, V., Vieu,
C., Thibault, C., Trevisiol, E., Francois, J.M. A comparative
study of α-Hemolysin expression in supported lipid bilayers of
synthetic and enriched complex bacterial lipid. BioNanoScience 4(2),
104-110 (2014). paper
supp
Sun, Z.Z., Yeung, E., Hayes, C.A., Noireaux, V.,
Murray, M.M. Linear DNA for rapid prototyping of synthetic
biological circuits in an Escherichia coli based TX-TL
cell-free system. ACS
Synthetic Biology 3(6), 387-397 (2014). paper
supp
Caschera, F., Noireaux, V. Synthesis of 2.3 mg/ml of
protein with an all Escherichia coli cell-free
transcription-translation system. Biochimie 99, 162-168 (2013). paper
supp
Siegal-Gaskins, D., Noireaux, V., Murray, R.M.
Biomolecular resource utilization in elementary cell-free gene
circuits. The Proceedings of
the IEEE American Control Conference (2013).
Sun, Z.Z., Hayes, C.A., Shin, J., Caschera, F., Murray,
M.M., Noireaux, V. Protocols for Implementing an Escherichia
coli Based TX-TL Cell-Free Expression System for Synthetic
Biology. J Vis Exp
(79), e50762 (2013). paper
supp
Shin, J., Jardine, P., Noireaux, V. Genome replication,
synthesis and assembly of the bacteriophage T7 in a single
cell-free reaction. ACS
Synthetic Biology 1(9), 408-413 (2012). paper
supp
Maeda, Y., Nakadai, T., Shin, J., Uryu, K., Noireaux,
V., Libchaber, A. Assembly of MreB filaments on vesicular
membranes: a synthetic biology approach. ACS Synthetic Biology
1(2), 53-59 (2012). paper
supp
Shin, J., Noireaux, V. An E. coli cell-free expression
toolbox: application to synthetic gene circuits and synthetic
cell. ACS Synthetic Biology
1(1), 29-41 (2012). paper
supp
Noireaux, V., Maeda, Y., Libchaber, L. Development of
an artificial cell, from self-organization to computation and
self-reproduction. Proc.
Nat. Acad. Sci. USA 108(9), 3473-3480 (2011). paper
Karzbrun, E., Shin, J., Bar-Ziv, R., Noireaux, V.
Coarse-grained dynamics of protein synthesis in a cell-free
system. Phys. Rev. Letters
106(4), 048104 (2011). paper
supp
Chalmeau, J., Monina, N., Shin, J., Vieu, C., Noireaux,
V. Alpha-hemolysin pore formation into a supported
phospholipid bilayer using cell-free expression. Biochim Biophys ActA 1808,
271-278 (2011). paper
supp
Shin, J., Noireaux, V. Study of messenger RNA
inactivation and protein degradation in an Escherichia coli
cell-free expression system. J.
Biol. Eng. 4:9 (2010). paper
supp
Shin, J., Noireaux, V. Efficient cell-free expression
with the endogenous E. Coli RNA polymerase and sigma factor
70. J. Biol. Eng. 4:8
(2010). paper
supp
Noireaux, V., Bar-Ziv, R., Godefroy, J., Salman, S.,
Libchaber, A. Toward an artificial cell based on gene
expression in vesicles. Phys.
Biol. 2, 1-8 (2005). paper
supp
Noireaux, V., Libchaber, A. A vesicle bioreactor as a
step toward an artificial cell assembly. Proc. Nat. Acad. Sci. USA
101, 17669-17674 (2004). paper
Noireaux, V., Bar-Ziv, R., Libchaber, A. Principles of
cell-free genetic circuit assembly. Proc. Nat. Acad. Sci. USA 100, 12672-12677
(2003). paper
Boukellal, H., Plastino, J., Noireaux, V., Sykes, C.
Propelling soft objects. CR
PHYS 4, 275-280 (2003). paper
Dubertret, B., Skourides, P., Norris, D.J., Noireaux,
V., Brivanlou, A.H., Libchaber, A. In vivo imaging of quantum
dots encapsulated in phospholipids micelles. Science 298, 1759-1762
(2002). paper
supp
Fradelizi, J., Noireaux, V., Plastino, J., Menichi, B.,
Louvard, D., Sykes, C., Golsteyn, R.M., Friederich, E. ActA
and the human Zyxin harbour Arp2/3 complex independent actin
polymerisation activity. Nat.
Cell Biol. 3, 699-707 (2001). paper
Noireaux, V., Golsteyn, R.M., Friederich, E., Prost,
J., Antony, C., Louvard, D., Sykes, C. Growing an actin gel on
spherical surfaces. Biophy.
J. 78, 1643-1654 (2000). paper
Plastino, J., Noireaux, V., Louvard, D., Antony, C.,
Sykes, C., Golsteyn, R.M. Dynamic cellular actin
polymerization in vitro. Recent
Res. Devel. Biophys. Chem. 1, 15-25 (2000).
Gerbal, F., Noireaux, V., Sykes, C., Julicher, F.,
Chaikin, P., Ott, A., Prost, J., Golsteyn, R.M., Freiderich,
E., Louvard, D., Laurent, V., Carlier, M.F. On the Listeria
propulsion mechanism. PRAMANA-J.Phys.
53, 155-170 (1999). paper
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